Multispectral Imaging (MSI) datasets were acquired using IRIS II which is a lightweight portable system comprising of a high resolution camera, a novel filter wheel able to interchange 23 filter positions and fast electronics and upon the same subject and viewing conditions. The wavelengths that the 23 images of each dataset were acquired are the following: 360nm, 370nm, 380nm, 400nm, 425nm, 475nm, 500nm, 525nm, 550nm, 575nm, 600nm, 625nm, 650nm, 700nm,750nm, 800nm, 850nm, 900nm, 950nm, 1000nm, 1050nm, 1100nm and 1150nm. The landmarks establishing ground truth correspondences across all images of the sequence were markers. In particular, = 16 small markers were printed using a laser printer on white paper. Each target was a, square, small, black and white 2 x 2 checkerboard, which was clearly distinguishable in each wavelength. The targets were placed in a 4 x 4 grid arrangement covering almost entirely the field of view, as shown in the following figure. Marker size was 25mm2 and markers were imaged by approximately 40x40 pixels. The images are encoded in 16bits, in the PNG image file format. More details about the acquisition of the datasets and the imaged targets can be found in the above paper.